Anticorps Monoclonal anti-GSDMD

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(52)

CloneNo.

2E6C7

N° de cat : 66387-1-Ig

Synonymes

2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

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Galerie de données de validation

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  WB analysis using 66387-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66387-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 66387-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66387-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66387-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 66387-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of TF-1 using 66387-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66387-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of EC109 using 66387-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66387-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66387-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 66387-1-Ig

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 66387-1-Ig

  IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug.

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  IF Staining of HeLa using 66387-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (66387-1-Ig, Clone: 2E6C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules A549, cellules COLO 320, cellules EC109, cellules HepG2, cellules HL-60, cellules Jurkat, cellules K-562, cellules PC-3, cellules Raji, cellules TF-1, cellules U2OS, tissu de côlon humain, tissu d'intestin grêle humain
• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 47 publications below
• IHC: See 4 publications below
• IF: See 13 publications below

Informations sur le produit

66387-1-Ig cible GSDMD dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSDMD Protéine recombinante Ag16827
• Nom complet: gasdermin D
• Masse moléculaire calculée: 484 aa, 53 kDa
• Poids moléculaire observé: 47-53 kDa
• Numéro d’acquisition GenBank: BC008904
• Symbole du gène: GSDMD
• Identification du gène (NCBI): 79792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

Publications

Species	Application	Title
mouse	WB	Mol Cancer; Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases; Authors - Qiang Wang
human	WB	PLoS Pathog; An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).; Authors - Lan Lin
human	WB	iScience; ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1; Authors - Ozlem Calbay
mouse	WB	Biochem Pharmacol; Deficiency of purinergic P2X4 receptor alleviates experimental autoimmune hepatitis in mice; Authors - Zejin Liu
human	WB	Emerg Microbes Infect; Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.; Authors - Lei Xu
mouse	WB	Mol Med; MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription; Authors - Nan Zhao

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