Anticorps Monoclonal anti-GSDMD

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

2E6C7

N° de cat : 66387-1-PBS

Synonymes

2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

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Galerie de données de validation

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  WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66387-1-Ig (same clone as 66387-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of EC109 using 66387-1-Ig (same clone as 66387-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66387-1-Ig (same clone as 66387-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66387-1-Ig (same clone as 66387-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of human small intestine using 66387-1-Ig (same clone as 66387-1-PBS)

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 66387-1-Ig (same clone as 66387-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66387-1-Ig (same clone as 66387-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66387-1-Ig (same clone as 66387-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66387-1-Ig (same clone as 66387-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  WB analysis of TF-1 using 66387-1-Ig (same clone as 66387-1-PBS)

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

  IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (66387-1-Ig, Clone: 2E6C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66387-1-PBS cible GSDMD dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSDMD Protéine recombinante Ag16827
• Nom complet: gasdermin D
• Masse moléculaire calculée: 484 aa, 53 kDa
• Poids moléculaire observé: 47-53 kDa
• Numéro d’acquisition GenBank: BC008904
• Symbole du gène: GSDMD
• Identification du gène (NCBI): 79792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

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