Anticorps Recombinant de lapin anti-GSK3B

GSK3B Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4N21

N° de cat : 82061-1-PBS

Synonymes

GSK3 beta, 4N21, EC:2.7.11.1, EC:2.7.11.26, GSK 3 beta

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Galerie de données de validation

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  WB analysis using 82061-1-RR (same clone as 82061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82061-1-RR (GSK3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82061-1-RR (same clone as 82061-1-PBS)

  IP result of anti-GSK3B (IP:82061-1-RR, 4ug; Detection:82061-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82061-1-PBS cible GSK3B dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GSK3B Protéine recombinante Ag17320
• Nom complet: glycogen synthase kinase 3 beta
• Masse moléculaire calculée: 433 aa, 48 kDa
• Poids moléculaire observé: ~45 kDa
• Numéro d’acquisition GenBank: BC000251
• Symbole du gène: GSK3B
• Identification du gène (NCBI): 2932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.

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