Anticorps Monoclonal anti-GSS

• Phare
• Validé par KD/KO

GSS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris, Hamster

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F12G1

N° de cat : 67598-1-PBS

Synonymes

1F12G1, EC:6.3.2.3, Glutathione synthase, glutathione synthetase, GSH S

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Galerie de données de validation

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  WB analysis using 67598-1-Ig (same clone as 67598-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67598-1-Ig (same clone as 67598-1-PBS)

  WB result of GSS antibody (67598-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  WB analysis using 67598-1-Ig (same clone as 67598-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67598-1-Ig (GSS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67598-1-Ig (GSS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67598-1-Ig (same clone as 67598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSS antibody (67598-1-Ig, Clone: 1F12G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67598-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67598-1-PBS cible GSS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris, Hamster

• Réactivité: Humain, porc, rat, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSS Protéine recombinante Ag8354
• Nom complet: glutathione synthetase
• Masse moléculaire calculée: 474 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007927
• Symbole du gène: GSS
• Identification du gène (NCBI): 2937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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