Anticorps Monoclonal anti-GST Tag

GST Tag Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Protéine recombinante, schistosoma japonicum et plus (2)

Applications

WB, IP, CoIP

Conjugaison

HRP

Publications

34

CloneNo.

3G12B10

N° de cat : HRP-66001

Synonymes

GST, GST tag

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Galerie de données de validation

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  WB analysis of Recombinant protein using HRP-66001

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66001 (GST Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

HRP-66001 cible GST Tag dans les applications de WB, IP, CoIP et montre une réactivité avec des échantillons Protéine recombinante, schistosoma japonicum

• Réactivité: Protéine recombinante, schistosoma japonicum
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: GST Tag
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: -
• Numéro d’acquisition GenBank: FN315687
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

Species	Application	Title
		Nature; Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.; Authors - Zhaoyang Li
	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
		Cell Res; Degradation of lipid droplets by chimeric autophagy-tethering compounds.; Authors - Yuhua Fu
human	CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
	WB	Nat Commun; PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.; Authors - Shifan Wang
	WB	Redox Biol; The novel β-TrCP protein isoform hidden in circular RNA confers trastuzumab resistance in HER2-positive breast cancer; Authors - Shengting Wang

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