Anticorps Polyclonal de lapin anti-GSTA3

GSTA3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 16703-1-AP

Synonymes

GST class-alpha member 3, Glutathione S-transferase A3-3, Glutathione S-transferase A3, Glutathione S transferase A3 3, Glutathione S transferase A3

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Galerie de données de validation

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  WB analysis using 16703-1-AP

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 16703-1-AP (GSTA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16703-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16703-1-AP (GSTA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16703-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16703-1-AP (GSTA3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16703-1-AP

  IP result of anti-GSTA3 (IP:16703-1-AP, 4ug; Detection:16703-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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  IF Staining of MCF-7 using 16703-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GSTA3 antibody (16703-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

16703-1-AP cible GSTA3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTA3 Protéine recombinante Ag10101
• Nom complet: glutathione S-transferase alpha 3
• Masse moléculaire calculée: 222 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC020619
• Symbole du gène: GSTA3
• Identification du gène (NCBI): 2940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization.; Authors - Mohammad Nasiruddin Rana
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
mouse	WB	Am J Pathol; Novel Protective Role of Nicotinamide Phosphoribosyltransferase in Acetaminophen-Induced Acute Liver Injury in Mice.; Authors - Li Qin Zhang
rat	WB	PLoS One; Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells.; Authors - Yoko Ito
mouse	WB	J Toxicol Pathol; Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice.; Authors - Shirai Makoto M
human	IHC	Radiother Oncol; Transcriptomic and epigenetic landscape of nimorazole-enhanced radiochemotherapy in head and neck cancer; Authors - María José Besso

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