Anticorps Polyclonal de lapin anti-GSTA4

GSTA4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 17271-1-AP

Synonymes

EC:2.5.1.18, Glutathione S transferase A4, Glutathione S transferase A4 4, Glutathione S-transferase A4, Glutathione S-transferase A4-4

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Galerie de données de validation

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  WB analysis of human brain using 17271-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17271-1-AP (GSTA4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17271-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17271-1-AP (GSTA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 17271-1-AP

  IP result of anti-GSTA4 (IP:17271-1-AP, 4ug; Detection:17271-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

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  IF Staining of HEK-293 using 17271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 17271-1-AP (GSTA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

17271-1-AP cible GSTA4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTA4 Protéine recombinante Ag11028
• Nom complet: glutathione S-transferase alpha 4
• Masse moléculaire calculée: 222 aa, 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC015523
• Symbole du gène: GSTA4
• Identification du gène (NCBI): 2941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSTA4 (Glutathione S-Transferase Alpha 4) is an enzyme that plays a key role in detoxification and antioxidant defense. Its structure includes a GSH-binding site and a hydrophobic substrate-binding domain, functioning as a dimer. Dysregulation of GSTA4 is linked to diseases like cancer, neurodegeneration, and diabetes.

Publications

Species	Application	Title
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Free Radic Biol Med; Increased hepatocellular protein carbonylation in human end-stage alcoholic cirrhosis.; Authors - C T Shearn
mouse	WB	Mech Ageing Dev; WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus; Authors - Jiahui Liu
mouse	WB	J Lipid Res; Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.; Authors - Rebecca L Smathers
		PLoS One; Short term feeding of a high fat diet exerts an additive effect on hepatocellular damage and steatosis in liver-specific PTEN knockout mice.; Authors - Colin T Shearn
human	WB	Exp Mol Pathol; Dysregulation of antioxidant responses in patients diagnosed with concomitant Primary Sclerosing Cholangitis/Inflammatory Bowel Disease.; Authors - Colin T Shearn

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iru (Verified Customer) (10-04-2021)

The antibody works well

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Fallopian tube epithelial cells

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