Anticorps Polyclonal de lapin anti-GSTK1

• Phare
• Validé par KD/KO

GSTK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (3)

N° de cat : 14535-1-AP

Synonymes

DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1

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Galerie de données de validation

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  WB analysis of HepG2 using 14535-1-AP

  WB result of GSTK1 antibody (14535-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells.

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  WB analysis using 14535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14535-1-AP

  IP result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug.

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  IHC staining of human testis using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14535-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HUVEC, cellules L02, cellules Raji, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14535-1-AP cible GSTK1 dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTK1 Protéine recombinante Ag6036
• Nom complet: glutathione S-transferase kappa 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC050715
• Symbole du gène: GSTK1
• Identification du gène (NCBI): 373156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

Publications

Species	Application	Title
mouse	WB	J Proteome Res; Proteomic Analysis of Hearts from Akita Mice Suggests Increases in Soluble Epoxide Hydrolase and Antioxidative Programming are Key Changes in Early Stages of Diabetic Cardiomyopathy.; Authors - Dewey Shannamar S
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
human	WB	Exp Mol Pathol; Aberrant expression of redox regulatory proteins in patients with concomitant primary Sclerosing cholangitis/inflammatory bowel disease.; Authors - Dennis R Petersen
human	CoIP	FASEB J; The role of chemerin in the regulation of cGAS-STING pathway in gestational diabetes mellitus placenta; Authors - Xuan Zhou
human,rat	WB	Sci Total Environ; m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance; Authors - Jingyuan Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (08-15-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

GSTK1 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:14535-1-AP)

FH

SD (Verified Customer) (07-05-2024)

Very good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

GSTK1 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:14535-1-AP)

FH

Andrea (Verified Customer) (01-23-2023)

Strong and specific signal.

• Applications: Western Blot

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