Anticorps Polyclonal de lapin anti-GSTK1

• Phare
• Validé par KD/KO

GSTK1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14535-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 14535-1-AP (same clone as 14535-1-PBS)

  WB result of GSTK1 antibody (14535-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14535-1-AP (same clone as 14535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 14535-1-AP (same clone as 14535-1-PBS)

  IP result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14535-1-AP (same clone as 14535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14535-1-PBS cible GSTK1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTK1 Protéine recombinante Ag6036
• Nom complet: glutathione S-transferase kappa 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC050715
• Symbole du gène: GSTK1
• Identification du gène (NCBI): 373156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

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