Anticorps Polyclonal de lapin anti-GSTM1

• Phare
• Validé par KD/KO

GSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(26)

Avis (1)

N° de cat : 12412-1-AP

Synonymes

EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1

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Galerie de données de validation

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  WB analysis using 12412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12412-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12412-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 12412-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12412-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, foie de rat, tissu ovarien de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

12412-1-AP cible GSTM1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTM1 Protéine recombinante Ag3113
• Nom complet: glutathione S-transferase mu 1
• Masse moléculaire calculée: 181 aa, 21 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC024005
• Symbole du gène: GSTM1
• Identification du gène (NCBI): 2944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSTM1 belongs to the superfamily of glutathione-Stransferases (GSTs) of phase 2 enzymes that catalyze a number of distinct glutathione-dependent reactions. GSTM1 gene has been a natural candidate in studies of susceptibility to inflammatory airway diseases with environmental components because of the high prevalence of the null polymorphism and its role in detoxification (PMID: 22683820).

Publications

Species	Application	Title
mouse	WB	Immunity; Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.; Authors - Yujia Wang ; KO Validated
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
human	IHC	Acta Neuropathol; Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy.; Authors - Woutje M Berdowski
mouse	WB	Sci Adv; lncRNA-GM targets Foxo1 to promote T cell-mediated autoimmunity; Authors - Yali Chen
human	WB	Autophagy; ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.; Authors - Zhiqiang Deng
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Antibody works well at provided dilution in 1X TBST. Bands showed up around 25 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse liver tissue

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