Anticorps Polyclonal de lapin anti-GSTM1

• Phare
• Validé par KD/KO

GSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12412-1-PBS

Synonymes

EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1

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Galerie de données de validation

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  WB analysis using 12412-1-AP (same clone as 12412-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12412-1-AP (same clone as 12412-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 12412-1-AP (same clone as 12412-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12412-1-PBS cible GSTM1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTM1 Protéine recombinante Ag3113
• Nom complet: glutathione S-transferase mu 1
• Masse moléculaire calculée: 181 aa, 21 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC024005
• Symbole du gène: GSTM1
• Identification du gène (NCBI): 2944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GSTM1 belongs to the superfamily of glutathione-Stransferases (GSTs) of phase 2 enzymes that catalyze a number of distinct glutathione-dependent reactions. GSTM1 gene has been a natural candidate in studies of susceptibility to inflammatory airway diseases with environmental components because of the high prevalence of the null polymorphism and its role in detoxification (PMID: 22683820).

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