Anticorps Monoclonal anti-GSTM1

GSTM1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, poulet, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C5C2

N° de cat : 67862-1-PBS

Synonymes

GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18

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Galerie de données de validation

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  WB analysis of rat liver using 67862-1-Ig (same clone as 67862-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67862-1-Ig (same clone as 67862-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  WB analysis of chicken liver using 67862-1-Ig (same clone as 67862-1-PBS)

  chicken liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67862-1-Ig (same clone as 67862-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67862-1-Ig (same clone as 67862-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67862-1-Ig (same clone as 67862-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67862-1-Ig (same clone as 67862-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSTM1 antibody (67862-1-Ig, Clone: 3C5C2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67862-1-Ig (same clone as 67862-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GSTM1 (67862-1-Ig, Clone:3C5C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67862-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67862-1-PBS cible GSTM1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, poulet, rat, souris

• Réactivité: Humain, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GSTM1 Protéine recombinante Ag3113
• Nom complet: glutathione S-transferase mu 1
• Masse moléculaire calculée: 181 aa, 21 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC024005
• Symbole du gène: GSTM1
• Identification du gène (NCBI): 2944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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