Anticorps Polyclonal de lapin anti-GTF3C2

GTF3C2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27494-1-AP

Synonymes

TFIIIC110, TFIIIC BETA, TF3C-beta, TF3C beta, KIAA0011

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Galerie de données de validation

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  WB analysis using 27494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27494-1-AP (GTF3C2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27494-1-AP (GTF3C2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 27494-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27494-1-AP (GTF3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27494-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GTF3C2 antibody (27494-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27494-1-AP cible GTF3C2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTF3C2 Protéine recombinante Ag26666
• Nom complet: general transcription factor IIIC, polypeptide 2, beta 110kDa
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC020981
• Symbole du gène: GTF3C2
• Identification du gène (NCBI): 2976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

Publications

Species	Application	Title
human	WB	Hum Genet; Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder; Authors - Aiko Iwata-Otsubo

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