Anticorps Polyclonal de lapin anti-GTF3C4

GTF3C4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17653-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug. This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17653-1-AP (same clone as 17653-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17653-1-AP (same clone as 17653-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17653-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17653-1-PBS cible GTF3C4 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTF3C4 Protéine recombinante Ag11898
• Nom complet: general transcription factor IIIC, polypeptide 4, 90kDa
• Masse moléculaire calculée: 822 aa, 92 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC094774
• Symbole du gène: GTF3C4
• Identification du gène (NCBI): 9329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.

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