Anticorps Polyclonal de lapin anti-GTPBP4

• Phare
• Validé par KD/KO

GTPBP4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13897-1-PBS

Synonymes

NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4

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Galerie de données de validation

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  WB analysis using 13897-1-AP (same clone as 13897-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 13897-1-AP (same clone as 13897-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13897-1-AP (same clone as 13897-1-PBS)

  IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:5000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

  IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 13897-1-AP (same clone as 13897-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using GTPBP4 antibody (13897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13897-1-PBS cible GTPBP4 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GTPBP4 Protéine recombinante Ag4859
• Nom complet: GTP binding protein 4
• Masse moléculaire calculée: 634 aa, 74 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC038975
• Symbole du gène: GTPBP4
• Identification du gène (NCBI): 23560
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.

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