Anticorps Polyclonal de lapin anti-Uroguanylin

Uroguanylin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 18113-1-AP

Synonymes

GUCA2B, GCAP II, GCAP II Uroguanylin, Guanylate cyclase activator 2B, Guanylate cyclase C-activating peptide 2

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Galerie de données de validation

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  WB analysis of mouse pancreas using 18113-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 18113-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 18113-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 18113-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 18113-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse small intestine using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18113-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18113-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Uroguanylin antibody (18113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu de côlon de souris, tissu d'intestin grêle de souris, tissu pancréatique de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain, tissu d'estomac humain, tissu d'intestin grêle de souris, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

18113-1-AP cible Uroguanylin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Uroguanylin Protéine recombinante Ag12589
• Nom complet: guanylate cyclase activator 2B (uroguanylin)
• Masse moléculaire calculée: 112 aa, 12 kDa
• Poids moléculaire observé: 12-13 kDa
• Numéro d’acquisition GenBank: BC093779
• Symbole du gène: Uroguanylin
• Identification du gène (NCBI): 2981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Uroguanylin, also named as GUCA2B, is a candidate intestinal natriuretic hormone that controls the sodium and water balance between the intestine and the kidneys. It has been reported that the uroguanylin knockout mouse exhibits elevated blood pressure. Therefore, the GUCA2B gene is thought to be a susceptibility gene for essential hypertension (EH).

Publications

Species	Application	Title
mouse	IHC	Front Physiol; Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress.; Authors - Nobuhiko Ebisutani
human	WB	Hum Gene Ther; Gene Co-Expression and miRNA Regulation: A Path to Early Intervention in Colorectal Cancer; Authors - Jason C Huang
rat	IF	PLoS One; Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine; Authors - Natchayaporn Thonapan
human,mouse	WB,IF	Front Pharmacol; Brain-derived uroguanylin as a regulator of postprandial brown adipose tissue activation: a potential therapeutic approach for metabolic disorders; Authors - Nikola Habek

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