Anticorps Polyclonal de lapin anti-GUCY2D

GUCY2D Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 55127-1-AP

Synonymes

CORD5, CORD6, CYGD, Guanylate cyclase 2D, retinal, GUC1A4, GUC2D, GUCY2D, LCA, LCA1, retGC, RETGC 1, RETGC1, Retinal guanylyl cyclase 1, ROS GC, ROS GC1

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Galerie de données de validation

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  WB analysis of mouse brain using 55127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55127-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55127-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

55127-1-AP cible GUCY2D dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: guanylate cyclase 2D, membrane (retina-specific)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 120 kDa, 95 kDa
• Numéro d’acquisition GenBank: NM_000180
• Symbole du gène: GUCY2D
• Identification du gène (NCBI): 3000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GUCY2D, also named as CORD6, GUC1A4, GUC2D, RETGC, RETGC1 and ROS-GC, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. A number of nonsense and frameshift mutations in the GUCY2D gene have been identified in LCA1 patients. Defects in GUCY2D are the cause of cone-rod dystrophy type 6 (CORD6). GUCY2D is responsible for many reported cases of autosomal dominant CRDs. The antibody is specific to GUCY2D.

Publications

Species	Application	Title
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta

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