Anticorps Recombinant de lapin anti-GUF1

GUF1 Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251449D6

N° de cat : 86747-1-PBS

Synonymes

EC:3.6.5.-, EF-4, Elongation factor 4 homolog, GTPase GUF1, Ribosomal back-translocase

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Galerie de données de validation

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  WB analysis using 86747-1-RR (same clone as 86747-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86747-1-RR (GUF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86747-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 86747-1-RR (same clone as 86747-1-PBS)

  IP result of anti-GUF1 (IP:86747-1-RR, 4ug; Detection:86747-1-RR 1:2000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 86747-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86747-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86747-1-RR against Human GUF1 were performed. The affinity constant is 9.10 pM.

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Informations sur le produit

86747-1-PBS cible GUF1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GUF1 Protéine recombinante Ag11579
• Nom complet: GUF1 GTPase homolog (S. cerevisiae)
• Masse moléculaire calculée: 669 aa, 74 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC036768
• Symbole du gène: GUF1
• Identification du gène (NCBI): 60558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes.

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