Anticorps Monoclonal anti-GUK1

• Phare
• Validé par KD/KO

GUK1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2H5E1

N° de cat : 67047-1-Ig

Synonymes

GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 67047-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67047-1-Ig

  WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 67047-1-Ig

  WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 67047-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 67047-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67047-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67047-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67047-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 67047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 67047-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human thyroid cancer using 67047-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human thyroid cancer using 67047-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer de la thyroïde humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67047-1-Ig cible GUK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GUK1 Protéine recombinante Ag28805
• Nom complet: guanylate kinase 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-27 kDa
• Numéro d’acquisition GenBank: BC009914
• Symbole du gène: GUK1
• Identification du gène (NCBI): 2987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.