Anticorps Monoclonal anti-GUK1

• Phare
• Validé par KD/KO

GUK1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H5E1

N° de cat : 67047-1-PBS

Synonymes

EC:2.7.4.8, GMK, GMP kinase, GMPK, Guanylate kinase

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Galerie de données de validation

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  WB analysis of Jurkat using 67047-1-Ig (same clone as 67047-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67047-1-Ig (same clone as 67047-1-PBS)

  WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67047-1-Ig (same clone as 67047-1-PBS)

  WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HeLa cells. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67047-1-Ig (same clone as 67047-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67047-1-Ig (same clone as 67047-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 67047-1-Ig (same clone as 67047-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67047-1-Ig (same clone as 67047-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67047-1-Ig (same clone as 67047-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 67047-1-Ig (same clone as 67047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67047-1-Ig (same clone as 67047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 67047-1-Ig (same clone as 67047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  IF Staining of human thyroid cancer using 67047-1-Ig (same clone as 67047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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  IF Staining of human thyroid cancer using 67047-1-Ig (same clone as 67047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67047-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67047-1-PBS cible GUK1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GUK1 Protéine recombinante Ag28805
• Nom complet: guanylate kinase 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-27 kDa
• Numéro d’acquisition GenBank: BC009914
• Symbole du gène: GUK1
• Identification du gène (NCBI): 2987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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