Anticorps Polyclonal de lapin anti-GUSB

• Phare
• Validé par KD/KO

GUSB Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16332-1-AP

Synonymes

Beta G1, Beta glucuronidase, glucuronidase, beta, GUSB, MPS7

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Galerie de données de validation

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  WB analysis of mouse liver using 16332-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16332-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16332-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16332-1-AP (GUSB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16332-1-AP (GUSB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16332-1-AP (GUSB antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16332-1-AP (GUSB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 16332-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16332-1-AP (GUSB Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HL-60, cellules K-562, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

16332-1-AP cible GUSB dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GUSB Protéine recombinante Ag9453
• Nom complet: glucuronidase, beta
• Masse moléculaire calculée: 651 aa, 75 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC014142
• Symbole du gène: GUSB
• Identification du gène (NCBI): 2990
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The GUSB gene encodes beta-glucuronidase, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It is a tetrameric glycoprotein composed of identical subunits. GUSB plays an important role in the degradation of dermatan and keratan sulfates. GUSB has two isoforms with the molecular mass of 75 kDa and 69 kDa, and it always can be detected as 78 kDa, 60 kDa and 18 kDa. The 60 kDa and 18 kDa polypeptides are derived by nicking 78-kDa GUSB subunit at Val159-Gly160 (PMID: 1311180, 9268591).

Publications

Species	Application	Title
mouse	WB	Science; The adaptive immune system is a major driver of selection for tumor suppressor gene inactivation.; Authors - Timothy D Martin ; KO Validated
mouse	IHC	J Invest Dermatol; Distinct roles for Dectin-1 and Dectin-2 in skin wound healing and neutrophilic inflammatory responses.; Authors - Kenji Yamaguchi
human	WB	PLoS One; Changes in retinal pigment epithelium related to cigarette smoke: possible relevance to smoking as a risk factor for age-related macular degeneration.; Authors - Wang Ai Ling AL
mouse	WB	PLoS One; Mitochondrial dysfunction leads to deconjugation of quercetin glucuronides in inflammatory macrophages.; Authors - Ishisaka Akari A
mouse	WB	PLoS One; C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells.; Authors - Daiki Mori ; KO Validated