Anticorps Recombinant de lapin anti-Glycophorin A/CD235a

Glycophorin A/CD235a Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242822C9

N° de cat : 85182-1-PBS

Synonymes

Glycophorin A, 242822C9, CD235a, Glycophorin-A, GPA

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Galerie de données de validation

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  WB analysis of human placenta using 85182-1-RR (same clone as 85182-1-PBS)

  Human placenta tissue was subjected to SDS PAGE, followed by western blot with 85182-1-RR (GYPA antibody) at a dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85182-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85182-1-RR (same clone as 85182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-1-RR (Glycophorin A/CD235a antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85182-1-RR (same clone as 85182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-1-RR (Glycophorin A/CD235a antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01888-2

  Cytometric bead array standard curve of MP01888-2, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-1-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

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Informations sur le produit

85182-1-PBS cible Glycophorin A/CD235a dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Glycophorin A/CD235a Protéine recombinante Eg2336
• Nom complet: glycophorin A (MNS blood group)
• Masse moléculaire calculée: 150 aa, 16 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC005319
• Symbole du gène: Glycophorin A
• Identification du gène (NCBI): 2993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). Glycophorin A represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

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