Anticorps Polyclonal de lapin anti-GYPC

GYPC Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18147-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human blood using 18147-1-AP (same clone as 18147-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 18147-1-AP (GYPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18147-1-AP (same clone as 18147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18147-1-AP (GYPC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 18147-1-AP (same clone as 18147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using GYPC antibody (18147-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 18147-1-AP (same clone as 18147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GYPC antibody (18147-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18147-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18147-1-PBS cible GYPC dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GYPC Protéine recombinante Ag12825
• Nom complet: glycophorin C (Gerbich blood group)
• Masse moléculaire calculée: 128 aa, 14 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC106051
• Symbole du gène: GYPC
• Identification du gène (NCBI): 2995
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.

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