Anticorps Polyclonal de lapin anti-GYS1

• Phare
• Validé par KD/KO

GYS1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10566-1-AP

Synonymes

glycogen synthase 1 (muscle), GSY, GYS, GYS1

---

Galerie de données de validation

• 

  WB analysis using 10566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 10566-1-AP

  IP Result of anti-GYS1 (IP:10566-1-AP, 4ug; Detection:10566-1-AP 1:300) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of human liver using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10566-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10566-1-AP (GYS1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 10566-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10566-1-AP (GYS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10566-1-AP

  Immunofluorescent analysis of HepG2 cells, using GYS1 antibody 10566-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 10566-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GYS1 antibody (10566-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer de la prostate humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10566-1-AP cible GYS1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GYS1 Protéine recombinante Ag0857
• Nom complet: glycogen synthase 1 (muscle)
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC007688
• Symbole du gène: GYS1
• Identification du gène (NCBI): 2997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
human	WB,IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu ; KD Validated
mouse	WB	Arterioscler Thromb Vasc Biol; Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.; Authors - Hongming Zhu
rat	WB	Metabolism; Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat; Authors - Zejian Liu
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse	WB,IF	J Lipid Res; Dynamic and differential regulation of proteins that coat lipid droplets in fatty liver dystrophic mice.; Authors - Hall Angela M AM