Anticorps Recombinant de lapin anti-GYS2

GYS2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242595F10

N° de cat : 85122-4-PBS

Synonymes

242595F10, EC:2.4.1.11, Glycogen synthase 2, glycogen synthase 2 (liver)

---

Galerie de données de validation

• 

  WB analysis using 85122-4-RR (same clone as 85122-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85122-4-RR (GYS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85122-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85122-4-RR (same clone as 85122-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide (positive control) and mouse skeletal muscle tissue slide (Negative control) using 85122-4-RR (GYS2 antibody) at dilution of 1:50000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85122-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85122-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85122-4-RR against Human GYS2 were performed. The affinity constant is 3.11 nM.

---

Informations sur le produit

85122-4-PBS cible GYS2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GYS2 Protéine recombinante Ag18012
• Nom complet: glycogen synthase 2 (liver)
• Masse moléculaire calculée: 703 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC126310
• Symbole du gène: GYS2
• Identification du gène (NCBI): 2998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GYS2 (Glycogen Synthase 2) is a key enzyme involved in the synthesis of glycogen in the liver. It catalyzes the addition of glucose molecules to the growing glycogen chain, playing a crucial role in maintaining glucose homeostasis. GYS2 is essential for liver glycogen synthesis, which acts as a critical energy reserve for maintaining blood glucose levels, especially during fasting or prolonged exercise. Impairment of GYS2 function can lead to glycogen storage disease type 0 (GSD-0), characterized by hypoglycemia and liver glycogen deficiency. The GYS2 is highest expressed in the liver, and its expression in liver was significantly higher than other tissues. (PMID:39150597; PMID:37574425)

{{ptg:RelatedPrimaryAntibodies}}