Anticorps Polyclonal de lapin anti-Granzyme B

Granzyme B Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 13588-1-AP

Synonymes

CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, Granzyme B, GZMB, HLP, SECT

---

Galerie de données de validation

• 

  WB analysis of HL-60 using 13588-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13588-1-AP (GZMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 13588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13588-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13588-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13588-1-AP (GZMB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13588-1-AP

  Immunofluorescent analysis of Hela cells, using GZMB antibody 13588-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of NK92 using 13588-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human Granzyme B (13588-1-AP) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HL-60
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: NK92,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 15 publications below
• IF: See 4 publications below

Informations sur le produit

13588-1-AP cible Granzyme B dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Granzyme B Protéine recombinante Ag3883
• Nom complet: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC030195
• Symbole du gène: GZMB
• Identification du gène (NCBI): 3002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

Publications

Species	Application	Title
human	IHC	J Am Acad Dermatol; Generalized bullous fixed drug eruption is distinct from Stevens-Johnson syndrome/toxic epidermal necrolysis by immunohistopathological features.; Authors - Yung-Tsu Cho
mouse	IHC	Biomaterials; In situ subcutaneously injectable thermosensitive PEG-PLGA diblock and PLGA-PEG-PLGA triblock copolymer composite as sustained delivery of bispecific anti-CD3 scFv T-cell/anti-EGFR Fab Engager (BiTEE).; Authors - Pu-Sheng Wei
mouse	IHC	Cancer Immunol Res; Peptide Blocking of PD-1/PD-L1 Interaction for Cancer Immunotherapy.; Authors - Chunlin Li
human	IHC	Oncogene; B7-H4 is increased in lung adenocarcinoma harboring EGFR-activating mutations and contributes to immunosuppression.; Authors - Youwei Lu
mouse	IHC	Clin Transl Med; c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.; Authors - Lan Zhang
human	IHC	J Invest Dermatol; EZH2 inhibitor enhances the STING agonist-induced antitumor immunity in melanoma.; Authors - Tianxiao Xu