Anticorps Monoclonal anti-Galectin-1

• Phare
• Validé par KD/KO

Galectin-1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3G10D2

N° de cat : CL594-60223

Synonymes

Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein

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Galerie de données de validation

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  IF Staining of human colon cancer using CL594-60223

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:1200.

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  IF Staining of human colon cancer using CL594-60223

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:1200.

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  IF Staining of Saos-2 using CL594-60223

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:200.

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  IF Staining of U2OS using CL594-60223

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite®594 Galectin-1 antibody (CL594-60223, Clone: 3G10D2 ) at dilution of 1:200.

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  FC experiment of U2OS using CL594-60223

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated Galectin-1 Monoclonal antibody (CL594-60223, Clone:3G10D2)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du côlon humain, cellules U2OS
• Résultats positifs en IF/ICC: Saos-2 cells, cellules U2OS
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60223 cible Galectin-1 dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Galectin-1 Protéine recombinante Ag2432
• Nom complet: lectin, galactoside-binding, soluble, 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC020675
• Symbole du gène: Galectin-1
• Identification du gène (NCBI): 3956
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

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