Anticorps Polyclonal de lapin anti-Galectin 3

• Phare
• Validé par KD/KO

Galectin 3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30450-1-PBS

Synonymes

Galectin-3, GAL3, Gal 3, CBP35, CBP 35

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Galerie de données de validation

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  WB analysis using 30450-1-AP (same clone as 30450-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30450-1-AP (Galectin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 30450-1-AP (same clone as 30450-1-PBS)

  WB result of Galectin 3 antibody (30450-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 30450-1-AP (same clone as 30450-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30450-1-AP (Galectin 3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30450-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30450-1-PBS cible Galectin 3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: lectin, galactoside-binding, soluble, 3
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 29-31 kDa
• Numéro d’acquisition GenBank: BC001120
• Symbole du gène: Galectin-3
• Identification du gène (NCBI): 3958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

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