Anticorps Recombinant de lapin anti-Galectin-3

Galectin-3 Recombinant Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242453H8

N° de cat : 84889-3-RR

Synonymes

Gal-3, Lgals3, 35 kDa lectin

---

Galerie de données de validation

• 

  IHC staining of mouse colon using 84889-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 84889-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84889-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84889-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84889-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84889-3-RR against Mouse Galectin-3 were performed. The affinity constant is 57.2 pM.

---

Applications testées

• Résultats positifs en IHC: tissu de côlon de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:10000-1:40000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84889-3-RR cible Galectin-3 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Galectin-3 Protéine recombinante Eg1232
• Nom complet: lectin, galactose binding, soluble 3
• Masse moléculaire calculée: 28 kDa
• Symbole du gène: Lgals3
• Identification du gène (NCBI): 16854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

{{ptg:RelatedPrimaryAntibodies}}