Anticorps Recombinant de lapin anti-Galectin-3

Galectin-3 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242453H5

N° de cat : 98335-3-RR

Synonymes

Gal-3, Lgals3, 242453H5, 35 kDa lectin

---

Galerie de données de validation

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse Galectin-3 Rabbit RecAb (98335-3-RR, Clone:242453H5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b. Cells were not fixed.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse Galectin-3 Rabbit RecAb (98335-3-RR, Clone:242453H5)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98335-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98335-3-RR against Mouse Galectin-3 were performed. The affinity constant is 49.5 pM.

---

Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris Balb/c,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98335-3-RR cible Galectin-3 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Galectin-3 Protéine recombinante Eg1232
• Nom complet: lectin, galactose binding, soluble 3
• Masse moléculaire calculée: 28 kDa
• Symbole du gène: Lgals3
• Identification du gène (NCBI): 16854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

{{ptg:RelatedPrimaryAntibodies}}