Anticorps Monoclonal anti-Gamma Catenin

Gamma Catenin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A5D10

N° de cat : 66445-1-PBS

Synonymes

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

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Galerie de données de validation

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  WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66445-1-Ig (same clone as 66445-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66445-1-Ig (same clone as 66445-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66445-1-Ig (same clone as 66445-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDCK using 66445-1-Ig (same clone as 66445-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66445-1-Ig (same clone as 66445-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66445-1-PBS cible Gamma Catenin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris

• Réactivité: canin, Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Gamma Catenin Protéine recombinante Ag1627
• Nom complet: junction plakoglobin
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC011865
• Symbole du gène: Gamma Catenin
• Identification du gène (NCBI): 3728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

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