Anticorps Monoclonal anti-Gelsolin

• Phare
• Validé par KD/KO

Gelsolin Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgA

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C4A2

N° de cat : 66280-1-PBS

Synonymes

3C4A2, Actin-depolymerizing factor, GSN

---

Galerie de données de validation

• 

  WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66280-1-Ig (same clone as 66280-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66280-1-Ig (same clone as 66280-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66280-1-PBS cible Gelsolin dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgA
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Gelsolin Protéine recombinante Ag17916
• Nom complet: gelsolin (amyloidosis, Finnish type)
• Masse moléculaire calculée: 782 aa, 86 kDa
• Poids moléculaire observé: 80-83 kDa
• Numéro d’acquisition GenBank: BC026033
• Symbole du gène: Gelsolin
• Identification du gène (NCBI): 2934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.

{{ptg:RelatedPrimaryAntibodies}}