Anticorps Monoclonal anti-Glucagon

Glucagon Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A8G1

N° de cat : 67286-1-PBS

Synonymes

GCG, 2A8G1, Glicentin-related polypeptide, Glucagon-like peptide 1, Glucagon-like peptide 1(7-36)

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Galerie de données de validation

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  IHC staining of human pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000(under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

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  IF Staining of human pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 67286-1-Ig (Glucagon antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The B cells of the islets of Langerhans was labelled in red with 15848-1-AP (Insulin antibody). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), INS antibody (15848-1-AP, red). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67286-1-PBS cible Glucagon dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glucagon Protéine recombinante Ag10629
• Nom complet: glucagon
• Masse moléculaire calculée: 180 aa, 21 kDa
• Numéro d’acquisition GenBank: BC005278
• Symbole du gène: Glucagon
• Identification du gène (NCBI): 2641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.

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