Anticorps Recombinant de lapin anti-Glucocorticoid receptor

Glucocorticoid receptor Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F22

N° de cat : 82619-4-PBS

Synonymes

NR3C1, GRL, GR, GCR, GCCR

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Galerie de données de validation

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  WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

  IP result of anti-Glucocorticoid receptor (IP:82619-4-RR, 4ug; Detection:82619-4-RR 1:8000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 82619-4-RR (same clone as 82619-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 82619-4-RR (same clone as 82619-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (82619-4-RR, Clone: 2F22 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82619-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82619-4-RR against Human Glucocorticoid receptor were performed. The affinity constant is 0.693 nM.

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Informations sur le produit

82619-4-PBS cible Glucocorticoid receptor dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Glucocorticoid receptor Protéine recombinante Ag21146
• Nom complet: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 94-97 kDa
• Numéro d’acquisition GenBank: BC015610
• Symbole du gène: Glucocorticoid receptor
• Identification du gène (NCBI): 2908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

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