Anticorps Monoclonal anti-Glucocorticoid receptor

Glucocorticoid receptor Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1D9A9

N° de cat : CL594-66904

Synonymes

NR3C1, 1D9A9, GCCR, GCR, GR

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Galerie de données de validation

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  IF Staining of mouse brain using CL594-66904

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 Glucocorticoid receptor antibody (CL594-66904, Clone: 1D9A9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using CL594-66904

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 Glucocorticoid receptor antibody (CL594-66904, Clone: 1D9A9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using CL594-66904

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Glucocorticoid receptor (CL594-66904, Clone:1D9A9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66904 cible Glucocorticoid receptor dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glucocorticoid receptor Protéine recombinante Ag28431
• Nom complet: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC015610
• Symbole du gène: Glucocorticoid receptor
• Identification du gène (NCBI): 2908
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

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