Anticorps Polyclonal de lapin anti-Glutamine Synthetase

• Phare
• Validé par KD/KO

Glutamine Synthetase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

N° de cat : 11037-2-PBS

Synonymes

GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase

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Galerie de données de validation

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  WB analysis of Jurkat using 11037-2-AP (same clone as 11037-2-PBS)

  WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  WB analysis using 11037-2-AP (same clone as 11037-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  IP result of anti-Glutamine Synthetase (IP:11037-2-AP, 4ug; Detection:11037-2-AP 1:5000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 11037-2-AP (same clone as 11037-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IF Staining of human brain using 11037-2-AP (same clone as 11037-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 11037-2-AP (same clone as 11037-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11037-2-PBS cible Glutamine Synthetase dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glutamine Synthetase Protéine recombinante Ag1510
• Nom complet: glutamate-ammonia ligase (glutamine synthetase)
• Masse moléculaire calculée: 374 aa, 42 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC011700
• Symbole du gène: Glutamine Synthetase
• Identification du gène (NCBI): 2752
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

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