Anticorps Polyclonal de lapin anti-GlyT2

GlyT2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25948-1-PBS

Synonymes

SLC6A5, Sodium- and chloride-dependent glycine transporter 2, NET1, GlyT-2, GlyT 2

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Galerie de données de validation

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  WB analysis using 25948-1-AP (same clone as 25948-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25948-1-AP (GlyT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25948-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 25948-1-AP (same clone as 25948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25948-1-AP (GlyT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25948-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25948-1-AP (same clone as 25948-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25948-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25948-1-AP (same clone as 25948-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25948-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25948-1-PBS cible GlyT2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GlyT2 Protéine recombinante Ag22835
• Nom complet: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
• Masse moléculaire calculée: 797 aa, 87 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC096319
• Symbole du gène: GlyT2
• Identification du gène (NCBI): 9152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GlyT2 is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. Like other members of the family, GlyT2 is trafficked to the cell surface as homo-oligomers from the endoplasmic reticulum (ER) (PMID: 32714574). GlyT2 is expressed in medulla, and to a lesser extent in spinal cord and cerebellum. GlyT2 has 3 isoforms with the molecular mass of 63, 86 and 87 kDa. The 63 kDa band is the unglycosylated GlyT2 polypeptide, whereas the 70-110 kDa band is a differential glycosylation form of GlyT2 (PMID: 17851090, 17962467).

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