Anticorps Monoclonal anti-Glycophorin A/CD235a

Glycophorin A/CD235a Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B12G10

N° de cat : 66778-1-PBS

Synonymes

Glycophorin A, 2B12G10, CD235a, Glycophorin-A, GPA

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Galerie de données de validation

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  WB analysis of human blood using 66778-1-Ig (same clone as 66778-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 66778-1-Ig (same clone as 66778-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66778-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50422-2

  Cytometric bead array standard curve of MP50422-2, GYPA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66778-2-PBS. Detection antibody: 66778-1-PBS. Standard:Ag8635. Range: 0.391-100 ng/mL.

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Informations sur le produit

66778-1-PBS cible Glycophorin A/CD235a dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Glycophorin A/CD235a Protéine recombinante Ag8635
• Nom complet: glycophorin A (MNS blood group)
• Masse moléculaire calculée: 150 aa, 16 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC005319
• Symbole du gène: Glycophorin A
• Identification du gène (NCBI): 2993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

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