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Anticorps Polyclonal de lapin anti-Glypican 3

Glypican 3 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25175-1-PBS

Synonymes

GPC3, DGSX, Glypican-3 alpha subunit, Glypican-3 beta subunit, GTR2 2

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of HepG2 cells using Glypican 3 Polyclonal antibody (25175-1-AP)

    WB analysis of HepG2 using 25175-1-AP (same clone as 25175-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Glypican 3 Polyclonal antibody (25175-1-AP)

    WB analysis of HEK-293 using 25175-1-AP (same clone as 25175-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

Informations sur le produit

25175-1-PBS cible Glypican 3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Glypican 3 Protéine recombinante Ag10129
Nom complet glypican 3
Masse moléculaire calculée 580 aa, 66 kDa
Poids moléculaire observé 66 kDa
Numéro d’acquisition GenBankBC035972
Symbole du gène GPC3
Identification du gène (NCBI) 2719
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).

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