Anticorps Recombinant de lapin anti-GM-CSF

GM-CSF Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

230286E11

N° de cat : 98014-1-PBS

Synonymes

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

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Galerie de données de validation

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  FC experiment of C57BL/6 CD3 T cells using 98014-1-RR (same clone as 98014-1-PBS)

  1x10^6 unstimulated or PMA and ionomycin in presence of protein transport inhibitors stimulated C57BL/6 CD3 T cells were intracellularly stained with 0.25 ug Anti-Mouse Gm-csf (98014-1-RR, Clone: 230286E11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cell were then stained with FITC Plus Anti-Mouse CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98014-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98014-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98014-1-RR against Human Gm-csf were performed. The affinity constant is 2.25 nM.

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Informations sur le produit

98014-1-PBS cible GM-CSF dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: colony stimulating factor 2 (granulocyte-macrophage)
• Numéro d’acquisition GenBank: NM-009969
• Symbole du gène: GM-CSF
• Identification du gène (NCBI): 12981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.

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