Anticorps Recombinant de lapin anti-GPNMB

GPNMB Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250309C11

N° de cat : 86874-1-PBS

Synonymes

Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 86874-1-RR (same clone as 86874-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

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  WB analysis using 86874-1-RR (same clone as 86874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02737-1

  Serum of six mice was measured. The GPNMB concentration of detected samples was determined to be 14.00 ng/mL with a range of 10.09-19.47 ng/mL.

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  Sandwich ELISA standard curve of MP02737-1

  Sandwich ELISA standard curve of MP02737-1, Mouse GPNMB Recombinant Matched Antibody Pair - PBS only. 86874-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2814. 86874-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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Informations sur le produit

86874-1-PBS cible GPNMB dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GPNMB Protéine recombinante Eg2814
• Nom complet: glycoprotein (transmembrane) nmb
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 90-100 kDa, 120kDa
• Numéro d’acquisition GenBank: NM_053110.4
• Symbole du gène: Gpnmb
• Identification du gène (NCBI): 93695
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

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