Anticorps Polyclonal de lapin anti-Progranulin/PGRN

Progranulin/PGRN Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29491-1-AP

Synonymes

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

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Galerie de données de validation

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  WB analysis using 29491-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29491-1-AP (Granulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29491-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29491-1-AP (Granulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules 4T1, cellules HEK-293, cellules HepG2, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7, cellules U-937, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

29491-1-AP cible Progranulin/PGRN dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Progranulin/PGRN Protéine recombinante Ag30723
• Nom complet: granulin
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC000324
• Symbole du gène: Granulin
• Identification du gène (NCBI): 2896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Publications

Species	Application	Title
mouse	IHC	Immun Inflamm Dis; Progranulin Plays a Protective Role in Pneumococcal Meningitis by Inhibiting Pyroptosis; Authors - Jingyao Wang

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