Anticorps Polyclonal de lapin anti-Granzyme B

Granzyme B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 13588-1-PBS

Synonymes

GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1

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Galerie de données de validation

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  WB analysis of HL-60 using 13588-1-AP (same clone as 13588-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13588-1-AP (GZMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13588-1-AP (Granzyme B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13588-1-AP (Granzyme B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13588-1-PBS cible Granzyme B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Granzyme B Protéine recombinante Ag3883
• Nom complet: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC030195
• Symbole du gène: Granzyme B
• Identification du gène (NCBI): 3002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

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