Anticorps Recombinant de lapin anti-Granzyme B

Granzyme B Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240761C6

N° de cat : 83763-5-RR

Synonymes

GZMB, CSP B, CGL1, CGL 1, CCPI

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Galerie de données de validation

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  WB analysis of NK-92 using 83763-5-RR

  NK-92 cells were subjected to SDS PAGE followed by western blot with 83763-5-RR (GZMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83763-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83763-5-RR against Human Granzyme B were performed. The affinity constant is 0.281 nM.

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Applications testées

• Résultats positifs en WB: NK-92 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83763-5-RR cible Granzyme B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC030195
• Symbole du gène: Granzyme B
• Identification du gène (NCBI): 3002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

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