Anticorps Polyclonal de lapin anti-GSDMD

GSDMD Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 30695-1-AP

Synonymes

AW558049, DF5L, Dfna5l, gasdermin D, Gsdmdc1

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Galerie de données de validation

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  WB analysis using 30695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 30695-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 30695-1-AP

  IP result of anti-Gsdmd (IP:30695-1-AP, 4ug; Detection:30695-1-AP 1:4000) with RAW 264.7 cells lysate 1400 ug.

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Applications testées

• Résultats positifs en WB: EL-4 cells, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IP: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

30695-1-AP cible GSDMD dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSDMD Protéine recombinante Ag33609
• Nom complet: gasdermin D
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 50-53 kDa, 43 kDa
• Numéro d’acquisition GenBank: BC029813
• Symbole du gène: Gsdmd
• Identification du gène (NCBI): 69146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can detect 53 kDa full-length GSDMD and 43 kDa fragment as described in the literature (PMID: 28392147, PMID: 28979266).

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; MANF inhibits NLRP3 inflammasome activation by competitively binding to DDX3X in paraquat-stimulated alveolar macrophages; Authors - Yi Pu
human	WB	Front Immunol; Comprehensive multiomics analysis identifies PYCARD as a key pyroptosis-related gene in osteoarthritis synovial macrophages; Authors - Zihao Yao

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