Anticorps Polyclonal de lapin anti-Phospho-GSK3B (Ser389)

Phospho-GSK3B (Ser389) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(49)

N° de cat : 14850-1-AP

Synonymes

GSK3B (Ser389), GSK3β (Ser389), GSK-3β (Ser389), p-GSK 3β (Ser389), p-GSK3B

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Galerie de données de validation

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  WB analysis using 14850-1-AP

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14850-1-AP (Phospho-GSK3B (Ser389) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 47 publications below
• IF: See 1 publications below

Informations sur le produit

14850-1-AP cible Phospho-GSK3B (Ser389) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glycogen synthase kinase 3 beta
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC060743
• Symbole du gène: Gsk3b
• Identification du gène (NCBI): 56637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase kinase 3s are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3α and GSK3β, encoded by separate genes, which are overall 85% homologous to each other, with 95% identity in the kinase domains. To date, phosphorylation at Ser9 by Akt is the best-characterized mechanism for the inhibition of GSK3β activity. GSK3β is phosphorylated at S389 in vivo by p38 MAPK and that this alternative regulatory mechanism of GSK3β is tissue-specific(PMID:18451303).

Publications

Species	Application	Title
mouse	WB	Redox Biol; Distinct dual roles of p-Tyr42 RhoA GTPase in tau phosphorylation and ATP citrate lyase activation upon different Aβ concentrations.; Authors - Kim Cuong Cap
human	WB	Cancer Res; p38 Stabilizes Snail by Suppressing DYRK2-Mediated Phosphorylation that is Required for GSK3β-βTrCP-Induced Snail Degradation.; Authors - Ki-Jun Ryu
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	Int J Mol Sci; GSK3β Serine 389 Phosphorylation Modulates Cardiomyocyte Hypertrophy and Ischemic Injury.; Authors - Laura Vainio
human	WB	Front Oncol; FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.; Authors - Yi-Wen Zheng
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong

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