Anticorps Polyclonal de lapin anti-Histone H2A.X

Histone H2A.X Polyclonal Antibody for WB, IHC, IF/ICC, ChIP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(152)

Avis (3)

N° de cat : 10856-1-AP

Synonymes

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

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Galerie de données de validation

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  WB analysis using 10856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10856-1-AP (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 10856-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10856-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Histone H2A.X antibody (10856-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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  IF Staining of MCF-7 using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H2A.X antibody (10856-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of U-251 using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using 10856-1-AP (Histone H2A.X antibody), at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  ChIP experiment of HEK-293 using 10856-1-AP

  Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 18 µg of cross-linked chromatin, 5 µg of Histone H2A Antibody (10856-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293, cellules HL-60, cœur de souris, rein de rat, rein de souris
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules U-251, cellules U2OS
• Résultats positifs en ChIP: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• CHImmunoprécipitation (IP): CHIP : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 112 publications below
• IHC: See 16 publications below
• IF: See 45 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10856-1-AP cible Histone H2A.X dans les applications de WB, IHC, IF/ICC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2A.X Protéine recombinante Ag1305
• Nom complet: H2A histone family, member X
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-18 kDa
• Numéro d’acquisition GenBank: BC013416
• Symbole du gène: Histone H2A.X
• Identification du gène (NCBI): 3014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

Publications

Species	Application	Title
human,mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
human	WB,IHC,IF	ACS Nano; Polyoxometalate-Based Radiosensitization Platform for Treating Hypoxic Tumors by Attenuating Radioresistance and Enhancing Radiation Response.; Authors - Yuan Yong
mouse	IF	ACS Nano; A Heterojunction Structured WO2.9-WSe2 Nanoradiosensitizer Increases Local Tumor Ablation and Checkpoint Blockade Immunotherapy upon Low Radiation Dose.; Authors - Xinghua Dong
mouse	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
mouse	IF	ACS Appl Mater Interfaces; Extremely Effective Chemoradiotherapy by Inducing Immunogenic Cell Death and Radio-Triggered Drug Release under Hypoxia Alleviation; Authors - Chunqi Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-27-2025)

Mouse liver lysates were subjected to SDS-PAGE and immunoblotted with Histone H2A.X Polyclonal antibody (1:2000 in 1XTBST). Bands appeared at 15 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue

Histone H2A.X Antibody Western Blot validation (1:2000 dilution) in Mouse Liver tissue (Cat no:10856-1-AP)

FH

Andreas (Verified Customer) (03-23-2022)

Antibody gives very good signal, hence the dilution factor. One important note is that I include 1:2000 dilution of benzonase in my lysis buffer to recover most of the chromatin-bound material, which big part of it is otherwise lost during the clarification step.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293FT

FH

kes (Verified Customer) (01-17-2022)

used for WB (1:1000) in milk+TBST buffer and got good results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LIver cell lines

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