Anticorps Polyclonal de lapin anti-Histone H2A.X

Histone H2A.X Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

98

Avis (2)

N° de cat : 10856-1-AP

Synonymes

H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, histone, Histone H2A.x

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Galerie de données de validation

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  WB analysis using 10856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10856-1-AP (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10856-1-AP (H2AFX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10856-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10856-1-AP (H2AFX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10856-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10856-1-AP (H2AFX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10856-1-AP (H2AFX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 10856-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10856-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10856-1-AP (Histone H2A.X antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 10856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using 10856-1-AP (Histone H2A.X antibody), at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10856-1-AP

  Immunofluorescent analysis of HepG2 cells, using H2AFX antibody 10856-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 10856-1-AP

  Immunofluorescent analysis of HepG2 cells, using H2AFX antibody 10856-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 10856-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Histone H2A.X antibody (10856-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293, cellules HL-60, cellules Raji, rein de rat, rein de souris, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2, cellules U-251
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 73 publications below
• IHC: See 7 publications below
• IF: See 28 publications below
• ChIP: See 1 publications below

Informations sur le produit

10856-1-AP cible Histone H2A.X dans les applications de WB, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2A.X Protéine recombinante Ag1305
• Nom complet: H2A histone family, member X
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-18 kDa
• Numéro d’acquisition GenBank: BC013416
• Symbole du gène: H2AFX
• Identification du gène (NCBI): 3014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

Publications

Species	Application	Title
human	WB,IHC,IF	ACS Nano; Polyoxometalate-Based Radiosensitization Platform for Treating Hypoxic Tumors by Attenuating Radioresistance and Enhancing Radiation Response.; Authors - Yuan Yong
mouse	IF	ACS Nano; A Heterojunction Structured WO2.9-WSe2 Nanoradiosensitizer Increases Local Tumor Ablation and Checkpoint Blockade Immunotherapy upon Low Radiation Dose.; Authors - Xinghua Dong
mouse	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
mouse	IF	ACS Appl Mater Interfaces; Extremely Effective Chemoradiotherapy by Inducing Immunogenic Cell Death and Radio-Triggered Drug Release under Hypoxia Alleviation; Authors - Chunqi Zhu
human	WB	Cancer Res; ZMYND8 Expression in Breast Cancer Cells Blocks T-Lymphocyte Surveillance to Promote Tumor Growth.; Authors - Yong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andreas (Verified Customer) (03-23-2022)

Antibody gives very good signal, hence the dilution factor. One important note is that I include 1:2000 dilution of benzonase in my lysis buffer to recover most of the chromatin-bound material, which big part of it is otherwise lost during the clarification step.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293FT

FH

kes (Verified Customer) (01-17-2022)

used for WB (1:1000) in milk+TBST buffer and got good results

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: LIver cell lines

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