Anticorps Polyclonal de lapin anti-Histone H2A.z

• Phare
• Validé par KD/KO

Histone H2A.z Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16441-1-AP

Synonymes

H2A histone family, member Z, H2A.z, H2A/z, H2AFZ, H2AZ, histone, Histone H2A, Histone H2A.Z

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Galerie de données de validation

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  WB analysis of human brain using 16441-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16441-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (Histone H2A.z Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16441-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

16441-1-AP cible Histone H2A.z dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, fish, Oratosquilla oratoria
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2A.z Protéine recombinante Ag9762
• Nom complet: H2A histone family, member Z
• Masse moléculaire calculée: 128 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC018002
• Symbole du gène: H2AFZ
• Identification du gène (NCBI): 3015
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
fish	IF	Sci Adv; The lungfish cocoon is a living tissue with antimicrobial functions.; Authors - Ryan Darby Heimroth
mouse	WB	J Biol Chem; The chromatin remodeler protein Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.; Authors - Haixin Zhao ; KD Validated
human	WB	Biomed Pharmacother; Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.; Authors - Ling Gao
mouse	IF,WB	Biol Open; Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway.; Authors - BreAnna Cameron
Oratosquilla oratoria	IF	J Morphol; Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda).; Authors - Tingrong Chen
human	WB	Front Oncol; DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway; Authors - Zhitao Han