Anticorps Monoclonal anti-HA Tag

HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1F5C6

N° de cat : 66006-2-PBS

Synonymes

YPYDVPDYA

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Galerie de données de validation

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  WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

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  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

  HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

  IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

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Informations sur le produit

66006-2-PBS cible HA Tag dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: HA Tag
• Symbole du gène: HA Tag
• Identification du gène (NCBI): 6
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

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