Anticorps Monoclonal anti-HABP2

HABP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7C2

N° de cat : CL488-67867

Synonymes

1A7C2, EC:3.4.21.-, Factor seven-activating protease, Factor VII activating protease, Factor VII-activating protease

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-67867

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HABP2 antibody (CL488-67867, Clone: 1A7C2 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-67867

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated HABP2 Monoclonal antibody (CL488-67867, Clone:1A7C2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67867 cible HABP2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HABP2 Protéine recombinante Ag3981
• Nom complet: hyaluronan binding protein 2
• Masse moléculaire calculée: 560 aa, 63 kDa
• Poids moléculaire observé: 60,63 kDa
• Numéro d’acquisition GenBank: BC031412
• Symbole du gène: HABP2
• Identification du gène (NCBI): 3026
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HABP2 is also named as HGFAL, PHBP and belongs to the peptidase S1 family. It can activate coagulation factor VII and prourokinase and release the vasoactive peptide bradykinin by cleaving kininogen and is an important role in the regulation of the haemostasis system as well as fibroproliferative inflammatory processes (PMID:21655671). HABP2 is present as the 70 kDa inactive single chain precursor in human plasma and transforms to the active two chain form, 50 kDa heavy chain and 27 kDa light chain,and the 50 kDa heavy chain changes to two 26 kDa fragments, and 27 kDa light chain to 17 kDa and 8 kDa fragments, which are all bridged by disulfide linkages, by autocleavage(PMID:11217080).

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